Journal: Iranian Journal of Public Health

Article Title: CircABCB10 Promotes the Apoptosis and Inflammatory Response of 16HBE Cells by Cigarette Smoke Extract by Targeting miR-130a/PTEN Axis

doi: 10.18502/ijph.v53i3.15141

Figure Lengend Snippet: Expression levels of circABCB10, miR-130a and PTEN in 16HBE cells after CSE treatment Note: A,16HBE cells were treated with CSE at different concentrations (0%, 0.5%, 1%, 2%, 2% and 4%) for 48h, and circABCB10 expression at the mRNA level was determined by qRT-PCR; B, Detection of PTEN expression in cells; C, Detection of miR-130a expression in cells; D–F, Gene expression of circABCB10, miR-130a and PTEN in 16HBE cells treated with 4% CSE at different time, respectively. ** represents P <0.01

Article Snippet: The cells used in this study were 16HBE human normal bronchial epithelial cell line (purchased from ATCC, USA).

Techniques: Expressing, Quantitative RT-PCR, Gene Expression

Journal: Iranian Journal of Public Health

Article Title: CircABCB10 Promotes the Apoptosis and Inflammatory Response of 16HBE Cells by Cigarette Smoke Extract by Targeting miR-130a/PTEN Axis

doi: 10.18502/ijph.v53i3.15141

Figure Lengend Snippet: miR-130a inhibitor can reverse the biological effect of knocking down circABCB10 Note: A, The expression of miR-130a was determined by qRT-PCR; B, The proliferation of 16HBE cells was determined by CCK8; C-D, The expression level of apoptosis protein c-caspase 3 and the protein ratio of c-caspase 3/ t-caspase 3 were detected by Western blot; E–G, The changes of expression levels of cytokines, IL-1β, IL-6 and TNF-α in the supernatant were determined by ELISA. ** represents P <0.01

Article Snippet: The cells used in this study were 16HBE human normal bronchial epithelial cell line (purchased from ATCC, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Iranian Journal of Public Health

Article Title: CircABCB10 Promotes the Apoptosis and Inflammatory Response of 16HBE Cells by Cigarette Smoke Extract by Targeting miR-130a/PTEN Axis

doi: 10.18502/ijph.v53i3.15141

Figure Lengend Snippet: circABCB10 was involved in the pathogenesis of COPD by regulating miR-130a/PTEN axis. Note: A, The mRNA level of PTEN were determined by qRT-PCR; B, The proliferation of 16HBE cells was determined by CCK8; C–D, The expression levels of the apoptotic protein c-caspase 3 and the protein ratio levels of ccaspase 3/T-caspase 3 were determined by Western blot; E–G, The changes of expression levels of cytokines, IL-1β, IL-6 and TNF-α in the supernatant of the medium were determined by ELISA. ** represents P <0.01

Article Snippet: The cells used in this study were 16HBE human normal bronchial epithelial cell line (purchased from ATCC, USA).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: Identification and validation of biomarkers related to mitophagy in chronic obstructive pulmonary disease

doi: 10.3892/mmr.2025.13458

Figure Lengend Snippet: MTERF3 expression is upregulated in CSE-treated 16HBE cells and knockdown of its expression suppresses the induction of apoptosis of 16HBE cells exposed to CSE. (A) CCK-8 assay was used to detect 16HBE cell viability with or with CSE exposure. (B) Immunoblotting was employed to assess MTERF3 expression in 16HBE cells with or with CSE exposure. ***P<0.001 vs. control group. (C) Immunoblotting was used to assess MTERF3 expression in 16HBE cells following transfection. **P<0.01 and ***P<0.001 vs. siRNA-NC group. (D) The CCK-8 assay was used to detect the viability of MTERF3-silenced 16HBE cells exposed to CSE. (E) Flow cytometric analysis was used to detect the induction of apoptosis of MTERF3-silenced 16HBE cells exposed to CSE. (F) Immunoblotting was used to assess the expression of apoptosis-related proteins in MTERF3-silenced 16HBE cells exposed to CSE. ***P<0.001 vs. control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. CSE + siRNA-NC group. MTERF3, mitochondrial transcription termination factor 3; CSE, cigarette smoke extract; CCK-8, Cell Counting Kit-8; cigarette smoke extract; siRNA, small interfering RNA; NC, negative control.

Article Snippet: The normal human bronchial epithelial cell line 16HBE was accessed from Procell Life Science & Technology Co., Ltd.

Techniques: Expressing, Knockdown, CCK-8 Assay, Western Blot, Control, Transfection, Cell Counting, Small Interfering RNA, Negative Control

Journal: Molecular Medicine Reports

Article Title: Identification and validation of biomarkers related to mitophagy in chronic obstructive pulmonary disease

doi: 10.3892/mmr.2025.13458

Figure Lengend Snippet: Knockdown of MTERF3 expression promotes mitophagy in CSE-treated 16HBE cells. (A) MDA content and (B) SOD activity were detected using the corresponding kits. (C) The generation of mitoROS was assessed with the application of Mito SOX Red staining. (D) MMP was evaluated using JC-1 staining. (E) Immunoblotting was employed to assess the expression of mitophagy-related proteins. ***P<0.001 vs. control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. CSE + siRNA-NC group. MTERF3, mitochondrial transcription termination factor 3; CSE, cigarette smoke extract; MDA, malondialdehyde; SOD, superoxide dismutase; MMP, matrix metalloproteinase; JC-1, 5,5′,6,6′-tetrachloro1,1′,3,3′-tetramethylbenzimidazolylcarbocyanine iodide; siRNA, small interfering RNA; NC, negative control.

Article Snippet: The normal human bronchial epithelial cell line 16HBE was accessed from Procell Life Science & Technology Co., Ltd.

Techniques: Knockdown, Expressing, Activity Assay, Staining, Western Blot, Control, Small Interfering RNA, Negative Control